Twist NGS portfolio provides exceptional performance, greater flexibility, and maximized sequencing efficiency, coming hand in hand with significant cost reduction. Years of IAB´ s hands-on experience assures that Twist solutions seamlessly integrate into your workflow.
Open a new era of your experiments with the tools of the future.
NGS Library Preparation Kits
Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results. Flexible DNA sample input from 1 ng – 1 μg (enzymatic) can be converted into robust, amplified libraries in under 2.5 hours.
Custom Panels
Twist Custom Panels can be designed and built to cover a wide range of panel sizes, target regions, and multiplexing requirements — all with exceptional and consistent performance. Whether you design your own panel from scratch or add targets to enhance the content of existing panels, you can use your Twist Custom Panel with Twist’s modular library preparation kits or seamlessly integrate them into your existing workflow. It is always possible to add content or enhance existing content easily.
Whole Exome Sequencing
Twist Exome 2.0 covers 36.5 Mb of human protein coding & non-coding regions. The content of Exome 2.0 is thoughtfully curated, based on recent database releases (Gencode, Refseq, CCDS, Ensembl), includes coding targets for COSMIC pathogenic variants, ACMG73 genes, complete genes with >500 pubmed citations, and more.
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IVDR Workflow for Whole Exome Sequencing
Twist’s Precision Dx Products present a whole exome sequencing library preparation and target enrichment workflow with IVDR certification. The suite includes Twist Precision Prep and Enrichment Dx Kit for library preparation and target enrichment, and the Twist Precision Exome Dx Panel with capture probes for exome enrichment. Additionally, you can order both these items together with Twist Precision Exome Dx Kit, which covers the whole library prep and exome enrichment workflow in one IVDR-compliant package.
cfDNA Library Preparation Kit
The Twist cfDNA Library Preparation Kit addresses challenges associated with library preparation from circulating cell-free DNA (cfDNA). Liquid biopsies have gained prominence in oncology research, particularly for investigating potential biomarkers within genetic material from tumors circulating in peripheral blood. This kit enables high conversion cfDNA library generation for next-generation sequencing (NGS) on Illumina systems, overcoming the technical challenges associated with low-input and degradation of cfDNA, as well as other samples that are hard to acquire and have limited DNA abundance (i.e. urine and CSF samples).
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Targeted Methylation Sequencing
The Twist Targeted Methylation System introduces a complete solution that produces highly complex and uniform sequencing reads for methylation analysis. The end-to-end protocol achieves this by combining an innovative, enzymatic conversion process, optimized target enrichment workflow, and highly developed panel design process. Twist Bioscience partnered with New England Biolabs to offer NEBNext® EMseq (enzymatic methyl-sequencing) library prep as part of the Twist Targeted Methylation System. A simple workflow modification enables secondary panels (or spike-ins) to be added to the methylome, useful when investigating new applications or areas of epigenetic research.
Twist RNA Solutions
Besides the Twist Whole Transcriptome RNAseq, Twist also offers transcriptome target enrichment with the Twist RNA Exome. Together with the Twist RNA Library Prep and Twist Target Enrichment, it provides a reliable method of generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the cell.
Synthetic Reference Material
The Twist cfDNA Pan-Cancer Reference Standard v2 is a comprehensive tool created to assist researchers in both the development and quality monitoring of clinically relevant variants for developing NGS-based assays. Additionally, researchers can utilize these reference standards to define two essential analytical parameters: the Limit of Detection (LoD) and the Limit of Blank (LoB) for their assays. Composed of wild-type (WT) background cell-free DNA (cfDNA) from a cell-line and synthetic oligos carrying mutant alleles, this reference material offers the precision and sensitivity needed for researching liquid biopsy assay development.